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Shanghai Model Organisms Center wild type littermates

SH3GLB1 deficiency protects against inflammation in ALI. (A) Gene expression of SH3GLB1 high AMs in cohort 1 (n=3) represented in a volcano plot, with the fold change (log2) expressed as the level in sham versus sea water-ALI subjects on the x-axis and the P-value on the y-axis (log10). Significantly (P<0.05) regulated genes are marked in colors: red indicates upregulated genes and blue indicates downregulated genes in ALI. (B) Representative GO analysis of SH3GLB1 high AMs. (C) Representative KEGG analysis of SH3GLB1 high AMs. (D) Representative hematoxylin and eosin staining of lung tissues (scale bars, 200 µ m). (E) Representative severity scores of lung injury (n=3). Representative ELISA results showing the levels of inflammatory factors, including (F) TNFα, (G) IL-6 and (H) IL-1β, in the BALF (n=6). (I-K) Representative ELISA analysis of the levels of inflammatory factors, including (I) IL-1β, (J) IL-6 and (K) TNFα, in the serum (n=6). (L) Representative western blots of SH3GLB1 protein levels in NC or SH3GLB1-KD BMDMs. (M) Relative SH3GLB1 mRNA levels in (NC or SH3GLB1-KD BMDMs. n=6/group. Representative ELISA analysis of the levels of inflammatory factors, including IL-1β, IL-6 and TNFα, in the supernatants of cells treated with (N) LPS or (O) seawater, (n=3).The data are presented as the means ± SEMs; P<0.05 was considered to indicate statistical significance. SH3GLB1, SH3 domain-containing GRB2-like protein B1; AMs, alveolar macrophages, ALI, acute lung injury; GO, Gene Ontology; KEGG, Kyoto Encyclopedia of Genes and Genomes; BALF, bronchoalveolar lavage fluid; BMDMs, bone marrow-derived macrophages; KD, knockdown; WT, <t>wild-type;</t> KO, knockout; NC, negative control; LPS, lipopolysaccharide.

Wild Type Littermates, supplied by Shanghai Model Organisms Center, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "Macrophage SH3 domain-containing GRB2-like protein B1 is required for Rab7-mediated mitophagy in inflammation during sea water drowning acute lung injury"

Article Title: Macrophage SH3 domain-containing GRB2-like protein B1 is required for Rab7-mediated mitophagy in inflammation during sea water drowning acute lung injury

Journal: International Journal of Molecular Medicine

doi: 10.3892/ijmm.2026.5809

Figure Legend Snippet: SH3GLB1 deficiency protects against inflammation in ALI. (A) Gene expression of SH3GLB1 high AMs in cohort 1 (n=3) represented in a volcano plot, with the fold change (log2) expressed as the level in sham versus sea water-ALI subjects on the x-axis and the P-value on the y-axis (log10). Significantly (P<0.05) regulated genes are marked in colors: red indicates upregulated genes and blue indicates downregulated genes in ALI. (B) Representative GO analysis of SH3GLB1 high AMs. (C) Representative KEGG analysis of SH3GLB1 high AMs. (D) Representative hematoxylin and eosin staining of lung tissues (scale bars, 200 µ m). (E) Representative severity scores of lung injury (n=3). Representative ELISA results showing the levels of inflammatory factors, including (F) TNFα, (G) IL-6 and (H) IL-1β, in the BALF (n=6). (I-K) Representative ELISA analysis of the levels of inflammatory factors, including (I) IL-1β, (J) IL-6 and (K) TNFα, in the serum (n=6). (L) Representative western blots of SH3GLB1 protein levels in NC or SH3GLB1-KD BMDMs. (M) Relative SH3GLB1 mRNA levels in (NC or SH3GLB1-KD BMDMs. n=6/group. Representative ELISA analysis of the levels of inflammatory factors, including IL-1β, IL-6 and TNFα, in the supernatants of cells treated with (N) LPS or (O) seawater, (n=3).The data are presented as the means ± SEMs; P<0.05 was considered to indicate statistical significance. SH3GLB1, SH3 domain-containing GRB2-like protein B1; AMs, alveolar macrophages, ALI, acute lung injury; GO, Gene Ontology; KEGG, Kyoto Encyclopedia of Genes and Genomes; BALF, bronchoalveolar lavage fluid; BMDMs, bone marrow-derived macrophages; KD, knockdown; WT, wild-type; KO, knockout; NC, negative control; LPS, lipopolysaccharide.

Techniques Used: Gene Expression, Staining, Enzyme-linked Immunosorbent Assay, Western Blot, Derivative Assay, Knockdown, Knock-Out, Negative Control

Restoration of SH3GLB1 expression provokes ALI. (A) Representative hematoxylin and eosin staining of lung tissues (scale bars, 100 µ m). (B) Representative severity scores of lung injury (n=3). Representative ELISA results showing the levels of inflammatory factors, including (C) TNFα, (D) IL-1β and (E) IL-6, in the BALF (n=6). Representative ELISA analysis of the levels of inflammatory factors, including (F) IL-1β, (G) IL-6 and (H) TNFα, in the serum (n=6). (I) Western blots of SH3GLB1 expression. (J) Relative SH3GLB1 mRNA levels in vectoror SH3GLB1-overexpressing BMDMs. n=6/group. Representative ELISA analysis of the levels of inflammatory factors, including IL-1β, IL-6 and TNFα, in the supernatants of cells treated with (K) LPS or (L) sea water (n=3).The data are presented as the means ± SEMs; P<0.05 was considered to indicate statistical significance. SH3GLB1, SH3 domain-containing GRB2-like protein B1; ALI, acute lung injury; BMDMs, bone marrow-derived macrophages; WT, wild-type; OV; overexpression.

Figure Legend Snippet: Restoration of SH3GLB1 expression provokes ALI. (A) Representative hematoxylin and eosin staining of lung tissues (scale bars, 100 µ m). (B) Representative severity scores of lung injury (n=3). Representative ELISA results showing the levels of inflammatory factors, including (C) TNFα, (D) IL-1β and (E) IL-6, in the BALF (n=6). Representative ELISA analysis of the levels of inflammatory factors, including (F) IL-1β, (G) IL-6 and (H) TNFα, in the serum (n=6). (I) Western blots of SH3GLB1 expression. (J) Relative SH3GLB1 mRNA levels in vectoror SH3GLB1-overexpressing BMDMs. n=6/group. Representative ELISA analysis of the levels of inflammatory factors, including IL-1β, IL-6 and TNFα, in the supernatants of cells treated with (K) LPS or (L) sea water (n=3).The data are presented as the means ± SEMs; P<0.05 was considered to indicate statistical significance. SH3GLB1, SH3 domain-containing GRB2-like protein B1; ALI, acute lung injury; BMDMs, bone marrow-derived macrophages; WT, wild-type; OV; overexpression.

Techniques Used: Expressing, Staining, Enzyme-linked Immunosorbent Assay, Western Blot, Derivative Assay, Over Expression

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Article Title: Macrophage SH3 domain‑containing GRB2‑like protein B1 is required for Rab7‑mediated mitophagy in inflammation during sea water drowning acute lung injury.
Article Snippet: .. A total of 20 SH3GLB1‐deficient mice on a C57BL/6J background and 20 of their wild‐type littermates were produced by Shanghai Model Organisms Center. ..

Article Title: Macrophage SH3 domain-containing GRB2-like protein B1 is required for Rab7-mediated mitophagy in inflammation during sea water drowning acute lung injury
Article Snippet: .. A total of 20 SH3GLB1-deficient mice on a C57BL/6J background and 20 of their wild-type littermates were produced by Shanghai Model Organisms Center. ..

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Journal: International Journal of Molecular Medicine

Article Title: Macrophage SH3 domain-containing GRB2-like protein B1 is required for Rab7-mediated mitophagy in inflammation during sea water drowning acute lung injury

doi: 10.3892/ijmm.2026.5809

Figure Lengend Snippet: SH3GLB1 deficiency protects against inflammation in ALI. (A) Gene expression of SH3GLB1 high AMs in cohort 1 (n=3) represented in a volcano plot, with the fold change (log2) expressed as the level in sham versus sea water-ALI subjects on the x-axis and the P-value on the y-axis (log10). Significantly (P<0.05) regulated genes are marked in colors: red indicates upregulated genes and blue indicates downregulated genes in ALI. (B) Representative GO analysis of SH3GLB1 high AMs. (C) Representative KEGG analysis of SH3GLB1 high AMs. (D) Representative hematoxylin and eosin staining of lung tissues (scale bars, 200 µ m). (E) Representative severity scores of lung injury (n=3). Representative ELISA results showing the levels of inflammatory factors, including (F) TNFα, (G) IL-6 and (H) IL-1β, in the BALF (n=6). (I-K) Representative ELISA analysis of the levels of inflammatory factors, including (I) IL-1β, (J) IL-6 and (K) TNFα, in the serum (n=6). (L) Representative western blots of SH3GLB1 protein levels in NC or SH3GLB1-KD BMDMs. (M) Relative SH3GLB1 mRNA levels in (NC or SH3GLB1-KD BMDMs. n=6/group. Representative ELISA analysis of the levels of inflammatory factors, including IL-1β, IL-6 and TNFα, in the supernatants of cells treated with (N) LPS or (O) seawater, (n=3).The data are presented as the means ± SEMs; P<0.05 was considered to indicate statistical significance. SH3GLB1, SH3 domain-containing GRB2-like protein B1; AMs, alveolar macrophages, ALI, acute lung injury; GO, Gene Ontology; KEGG, Kyoto Encyclopedia of Genes and Genomes; BALF, bronchoalveolar lavage fluid; BMDMs, bone marrow-derived macrophages; KD, knockdown; WT, wild-type; KO, knockout; NC, negative control; LPS, lipopolysaccharide.

Article Snippet: A total of 20 SH3GLB1-deficient mice on a C57BL/6J background and 20 of their wild-type littermates were produced by Shanghai Model Organisms Center.

Techniques: Gene Expression, Staining, Enzyme-linked Immunosorbent Assay, Western Blot, Derivative Assay, Knockdown, Knock-Out, Negative Control

Journal: International Journal of Molecular Medicine

Article Title: Macrophage SH3 domain-containing GRB2-like protein B1 is required for Rab7-mediated mitophagy in inflammation during sea water drowning acute lung injury

doi: 10.3892/ijmm.2026.5809

Figure Lengend Snippet: Restoration of SH3GLB1 expression provokes ALI. (A) Representative hematoxylin and eosin staining of lung tissues (scale bars, 100 µ m). (B) Representative severity scores of lung injury (n=3). Representative ELISA results showing the levels of inflammatory factors, including (C) TNFα, (D) IL-1β and (E) IL-6, in the BALF (n=6). Representative ELISA analysis of the levels of inflammatory factors, including (F) IL-1β, (G) IL-6 and (H) TNFα, in the serum (n=6). (I) Western blots of SH3GLB1 expression. (J) Relative SH3GLB1 mRNA levels in vectoror SH3GLB1-overexpressing BMDMs. n=6/group. Representative ELISA analysis of the levels of inflammatory factors, including IL-1β, IL-6 and TNFα, in the supernatants of cells treated with (K) LPS or (L) sea water (n=3).The data are presented as the means ± SEMs; P<0.05 was considered to indicate statistical significance. SH3GLB1, SH3 domain-containing GRB2-like protein B1; ALI, acute lung injury; BMDMs, bone marrow-derived macrophages; WT, wild-type; OV; overexpression.

Article Snippet: A total of 20 SH3GLB1-deficient mice on a C57BL/6J background and 20 of their wild-type littermates were produced by Shanghai Model Organisms Center.

Techniques: Expressing, Staining, Enzyme-linked Immunosorbent Assay, Western Blot, Derivative Assay, Over Expression

(A) Cartoon representation of Lamp2a knockout mouse generation. (B) Confocal microscopy imaging validating deletion of Lamp2a. Red staining reflects Lamp2a, green staining shows Lamp2b. Scale bar =300 µm (C) Immunoblot of RPE/choroid lysate showing loss of Lamp2a expression in Lamp2a -/- mice compared to wild type (WT) age matched mice. (D) FAF images showing high-intensity autofluorescence spots in WT and Lamp2a -/- mice. Lamp2a deficiency results in an age-dependent increase in high-intensity autofluorescence spots compared with WT mice. (E) Quantification of high-intensity autofluorescence spots (n=8). Statistical analysis was performed using Two-way ANOVA with Dunnett’s multiple comparisons test. ***P < 0.001; ****P < 0.0001 . (F) Retinal B-scans of Lamp2a -/- and WT mice. Significant structural disturbance in retinal layers was seen in 12 months Lamp2a -/- mice as compared to WT aged mice and Lamp2a -/- younger mice (n=6). Scale bar =100 µm (width) and 60 = µm (height). Values are expressed as mean ± SD. (G) Representative scotopic ERG response of 6-7-month-old WT and Lamp2a -/- mice. (H) b-wave quantification indicating decline in scotopic response in Lamp2a -/- mice relative to age-matched wild type (n=6 mice/group). (I) Representative scotopic response of 10-12-month-old WT and Lamp2a -/- mice. (J) B-wave quantification shows significant decline in scotopic response in Lamp2a -/- mice relative to age-matched WT (n=8 mice/group). (K) Representative maximal response of 6-7-month-old WT and Lamp2a -/- mice. (L) b-wave amplitude quantification shows decreased scotopic response in 6-7-month-old Lamp2a -/- mice relative to age-matched WT (n=6 mice/group). (M) Representative maximal response of 10-12 months old WT and Lamp2a -/- mice. (N) b-wave amplitude quantification shows significant decrease in scotopic response in 10-12-month-old Lamp2a -/- mice relative to age-matched wild type (n=8 mice/group). (O) Representative photopic response of 6-7-month-old WT and Lamp2a -/- mice. (P) b-wave amplitude quantification shows photopic response changes in 6-7-month-old Lamp2a -/- mice relative to age-matched WT (n=6 mice/group). (Q) Representative photopic response of 10-12-month-old WT and Lamp2a -/- mice. (R) b-wave amplitude quantification shows pronounced decrease in photopic response in 10-12-month-old Lamp2a -/- mice relative to age-matched WT (n=8 mice/group). Statistical differences were analyzed using an unpaired two-tailed student’s t-test. Level of significance was considered at p value < 0.05 .

Journal: bioRxiv

Article Title: Loss of Lamp2a-dependent chaperone-mediated autophagy drives dry AMD-like retinal pathology in mice and is rescued by BK channel activation

doi: 10.64898/2026.03.19.712761

Figure Lengend Snippet: (A) Cartoon representation of Lamp2a knockout mouse generation. (B) Confocal microscopy imaging validating deletion of Lamp2a. Red staining reflects Lamp2a, green staining shows Lamp2b. Scale bar =300 µm (C) Immunoblot of RPE/choroid lysate showing loss of Lamp2a expression in Lamp2a -/- mice compared to wild type (WT) age matched mice. (D) FAF images showing high-intensity autofluorescence spots in WT and Lamp2a -/- mice. Lamp2a deficiency results in an age-dependent increase in high-intensity autofluorescence spots compared with WT mice. (E) Quantification of high-intensity autofluorescence spots (n=8). Statistical analysis was performed using Two-way ANOVA with Dunnett’s multiple comparisons test. ***P < 0.001; ****P < 0.0001 . (F) Retinal B-scans of Lamp2a -/- and WT mice. Significant structural disturbance in retinal layers was seen in 12 months Lamp2a -/- mice as compared to WT aged mice and Lamp2a -/- younger mice (n=6). Scale bar =100 µm (width) and 60 = µm (height). Values are expressed as mean ± SD. (G) Representative scotopic ERG response of 6-7-month-old WT and Lamp2a -/- mice. (H) b-wave quantification indicating decline in scotopic response in Lamp2a -/- mice relative to age-matched wild type (n=6 mice/group). (I) Representative scotopic response of 10-12-month-old WT and Lamp2a -/- mice. (J) B-wave quantification shows significant decline in scotopic response in Lamp2a -/- mice relative to age-matched WT (n=8 mice/group). (K) Representative maximal response of 6-7-month-old WT and Lamp2a -/- mice. (L) b-wave amplitude quantification shows decreased scotopic response in 6-7-month-old Lamp2a -/- mice relative to age-matched WT (n=6 mice/group). (M) Representative maximal response of 10-12 months old WT and Lamp2a -/- mice. (N) b-wave amplitude quantification shows significant decrease in scotopic response in 10-12-month-old Lamp2a -/- mice relative to age-matched wild type (n=8 mice/group). (O) Representative photopic response of 6-7-month-old WT and Lamp2a -/- mice. (P) b-wave amplitude quantification shows photopic response changes in 6-7-month-old Lamp2a -/- mice relative to age-matched WT (n=6 mice/group). (Q) Representative photopic response of 10-12-month-old WT and Lamp2a -/- mice. (R) b-wave amplitude quantification shows pronounced decrease in photopic response in 10-12-month-old Lamp2a -/- mice relative to age-matched WT (n=8 mice/group). Statistical differences were analyzed using an unpaired two-tailed student’s t-test. Level of significance was considered at p value < 0.05 .

Article Snippet: Adult male Lamp2a knockout mice ( Lamp2a y/- ), female heterozygous Lamp2a +/- and their wild type (WT) littermates ( Lamp2a y/+ ) were obtained from Cyagen (Serial Number KOCMP-16784-Lamp2-B6J-VA; strain C57BL/6J-Lamp2 em1Cya ).

Techniques: Knock-Out, Confocal Microscopy, Imaging, Staining, Western Blot, Expressing, Two Tailed Test

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wild type littermates (Shanghai Model Organisms Center)

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